英语翻译Nitrite represents a circulating and tissue storage form

英语翻译
Nitrite represents a circulating and tissue storage form of NO whose bioactivation is mediated by the enzymatic action of xanthine oxidoreductase,nonenzymatic disproportionation,and reduction by deoxyhemoglobin,myoglobin,and tissue heme proteins.Because the rate of NO generation from nitrite is linearly dependent on reductions in oxygen and pH levels,we hypothesized that nitrite would be reduced to NO in ischemic tissue and exert NO-dependent protective effects.Solutions of sodium nitrite were administered in the setting of hepatic and cardiac ischemia-reperfusion (I/R) injury in mice.In hepatic I/R,nitrite exerted profound dose-dependent protective effects on cellular necrosis and apoptosis,with highly significant protective effects observed at near-physiological nitrite concentrations.In myocardial I/R injury,nitrite reduced cardiac infarct size by 67%.Consistent with hypoxia-dependent nitrite bioactivation,nitrite was reduced to NO,S-nitrosothiols,N-nitros-amines,and iron-nitrosylated heme proteins within 1-30 minutes of reperfusion.Nitrite-mediated protection of both the liver and the heart was dependent on NO generation and independent of eNOS and heme oxygenase-1 enzyme activities.These results suggest that nitrite is a biological storage reserve of NO subserving a critical function in tissue protection from ischemic injury.These studies reveal an unexpected and novel therapy for diseases such as myocardial infarction,organ preservation and transplantation,and shock states.

亚硝酸盐是一个循环和组织中一氧化氮的存储形式,其生物活化作用是由黄嘌呤氧化还原酶,非酶歧化和还原血红蛋白,肌红蛋白减少,并组织血红素蛋白质酶介导的行动.因为一氧化氮的生成率呈线性亚硝酸盐在氧气和pH值减少依赖性,我们推测亚硝酸盐将减少到缺血组织中一氧化氮和一氧化氮的产生依赖的保护作用.亚硝酸钠管理解决方案是在肝脏和心脏缺血再灌注（I / R）在小鼠损伤的设置.在肝I / R,亚硝酸盐产生深刻的剂量依赖性保护作用的细胞坏死和细胞凋亡的保护作用十分显着,观察在接近生理浓度的亚硝酸盐.在心肌I / R损伤,亚硝酸盐降低67％心肌梗死面积.缺氧依赖亚硝酸盐生物活化作用一致,亚硝酸盐减至不,第S -亚硝基的N-亚硝胺,并在1-30分钟,再灌注铁亚硝基血红素蛋白质.亚硝酸盐介导双方的肝脏和心脏的保护依赖于NO的生成及eNOS独立和血红素氧合酶1的活动.这些结果表明,亚硝酸盐是一种不促进从在组织缺血损伤保护重要功能的生物存储储备.这些研究揭示出一种如心肌梗死,器官保存和移植,疾病,意外和震惊国家和新颖的疗法.