英语翻译The first in vitro HCV infection of fresh monocytes/macr
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英语翻译
The first in vitro HCV infection of fresh monocytes/
macrophages was performed using cells isolated from
buffy-coats obtained from healthy donors [122].Negative
strands of HCV were usually found for up to 10
days after infection,while positive strands were found
for up to 27 days.Quasispecies analysis showed that
the complexity of the variants decreased after culturing,
and serum and monocytes/macrophages had different
major variants.Since monocytes/macrophages
isolated from blood could be infected by HCV,a follow-
up study could find no cytopathic effects or negative
strands in Kupffer cells obtained from liver cancer
patients Daudi cells have been investigated as a host for HCV
replication.One study showed that HCV in Daudi cells
can be detected for up to 19 days,and the total amount
of HCV increased 30-fold [124].They found that
enlarged Daudi cells were positive for HCV by ISH.
They also found virus-like particles about 42 nm in size
in the cytoplasm of cells displaying early signs of apoptosis.
Membrane bound organelles were common,as
were intracytoplasmic electron dense inclusion bodies.
Immature DC and mature DC were investigated as a
target of HCV [125].HCV positive strand,were detectable
for up to 10 days PI,while negative strands could
be seen as early as day 2.In mature DC,the positive
strand was detectable for only 5 days PI,and the negative
strands on days 1 and 2 PI.They also compared
quasispecies in the HVR1 of the inoculums and immature
DC positive strands at 6 days PI and found differences,
suggesting selection of particular variants by
immature DC.
The first in vitro HCV infection of fresh monocytes/
macrophages was performed using cells isolated from
buffy-coats obtained from healthy donors [122].Negative
strands of HCV were usually found for up to 10
days after infection,while positive strands were found
for up to 27 days.Quasispecies analysis showed that
the complexity of the variants decreased after culturing,
and serum and monocytes/macrophages had different
major variants.Since monocytes/macrophages
isolated from blood could be infected by HCV,a follow-
up study could find no cytopathic effects or negative
strands in Kupffer cells obtained from liver cancer
patients Daudi cells have been investigated as a host for HCV
replication.One study showed that HCV in Daudi cells
can be detected for up to 19 days,and the total amount
of HCV increased 30-fold [124].They found that
enlarged Daudi cells were positive for HCV by ISH.
They also found virus-like particles about 42 nm in size
in the cytoplasm of cells displaying early signs of apoptosis.
Membrane bound organelles were common,as
were intracytoplasmic electron dense inclusion bodies.
Immature DC and mature DC were investigated as a
target of HCV [125].HCV positive strand,were detectable
for up to 10 days PI,while negative strands could
be seen as early as day 2.In mature DC,the positive
strand was detectable for only 5 days PI,and the negative
strands on days 1 and 2 PI.They also compared
quasispecies in the HVR1 of the inoculums and immature
DC positive strands at 6 days PI and found differences,
suggesting selection of particular variants by
immature DC.
![英语翻译The first in vitro HCV infection of fresh monocytes/macr](/uploads/image/z/18293400-0-0.jpg?t=%E8%8B%B1%E8%AF%AD%E7%BF%BB%E8%AF%91The+first+in+vitro+HCV+infection+of+fresh+monocytes%2Fmacr)
首先在体外丙型肝炎病毒感染的单核细胞/
巨噬细胞是采用细胞分离
buffy-coats获得健康的捐助者[ 122].消极的
链的丙型肝炎病毒通常被发现为10
天感染后,虽然正链被发现
最多27天.准分析表明
复杂的变种减少后的培养,
和血清和单核/巨噬细胞有不同的
主要变种.由于单核/巨噬细胞
从血液中分离可能是感染了丙型肝炎病毒,跟随—
研究没有发现细胞病变效应或负
链在库普弗细胞从肝肿瘤
患者达乌迪恩细胞进行了调查作为一个主持人的丙型肝炎病毒
复制.一项研究表明,丙型肝炎病毒在达乌迪恩细胞
可以发现长达19天,和总金额
丙型肝炎病毒增加30倍[ 124].他们发现,
扩大的达乌迪恩细胞是积极的丙型肝炎病毒的原位杂交.
他们还发现病毒样颗粒约42纳米大小
在细胞质中的细胞早期凋亡的迹象显示.
膜结合细胞器是常见的,如
是胞浆内单电子致密包涵体.
未成熟和成熟的直流进行了调查作为一个
目标的丙型肝炎病毒[ 125].丙型肝炎病毒阳性,检出
长达10天,而负链可以
看到早在2天.成熟,积极
链是探测只有5天,与负
1日和2皮丝.他们还比较
准依次的接种和不成熟
直流正股在6天丕和发现的差异,
这意味着选择特定变种
未成熟的DC
巨噬细胞是采用细胞分离
buffy-coats获得健康的捐助者[ 122].消极的
链的丙型肝炎病毒通常被发现为10
天感染后,虽然正链被发现
最多27天.准分析表明
复杂的变种减少后的培养,
和血清和单核/巨噬细胞有不同的
主要变种.由于单核/巨噬细胞
从血液中分离可能是感染了丙型肝炎病毒,跟随—
研究没有发现细胞病变效应或负
链在库普弗细胞从肝肿瘤
患者达乌迪恩细胞进行了调查作为一个主持人的丙型肝炎病毒
复制.一项研究表明,丙型肝炎病毒在达乌迪恩细胞
可以发现长达19天,和总金额
丙型肝炎病毒增加30倍[ 124].他们发现,
扩大的达乌迪恩细胞是积极的丙型肝炎病毒的原位杂交.
他们还发现病毒样颗粒约42纳米大小
在细胞质中的细胞早期凋亡的迹象显示.
膜结合细胞器是常见的,如
是胞浆内单电子致密包涵体.
未成熟和成熟的直流进行了调查作为一个
目标的丙型肝炎病毒[ 125].丙型肝炎病毒阳性,检出
长达10天,而负链可以
看到早在2天.成熟,积极
链是探测只有5天,与负
1日和2皮丝.他们还比较
准依次的接种和不成熟
直流正股在6天丕和发现的差异,
这意味着选择特定变种
未成熟的DC
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