英语翻译CF release from native DPPC/DPPG-liposomes and POPC/POPG
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英语翻译
CF release from native DPPC/DPPG-liposomes and POPC/POPG-liposomes as well as liposome complexes withPLL,37C close to the transition temperature that is about 40C.This indicates that the DPPC/DPPG-liposomes being in the solid state have an impermeable membrane and complexation with PLL,leading to single covered vesicles or vesicle aggregates,doesnot disturb the integrity of the liposome membrane.
However,if the native or PLL-covered DPPC/DPPG-liposomes were heated to 54 °C (lipids are transferred to the fluid state with an accompanying reduction in the vesicle size,as was previously described) a significant release is observed (profiles 6 and 7,respectively).We propose that the higher membrane permeability of the liposomes in the fluid state compared to the solid is due to the presence of defects in the fluid membrane or to a transient fragmentation that must occur during the vesicle size reduction.The observed different permeability is in agreement with studies on DPPC-based vesicle ion permeability in different phase states [39].Our
experimentsreveal that PLL-covered“fluid”vesicles release the dyefaster,indicating a direct effect of bound PLL molecules on membrane permeability (Fig.5A,profile 7).At the same time we suppose that PLL molecules do not translocate across lipid membrane because just short polypeptides are able to cross the membranes .
CF release from native DPPC/DPPG-liposomes and POPC/POPG-liposomes as well as liposome complexes withPLL,37C close to the transition temperature that is about 40C.This indicates that the DPPC/DPPG-liposomes being in the solid state have an impermeable membrane and complexation with PLL,leading to single covered vesicles or vesicle aggregates,doesnot disturb the integrity of the liposome membrane.
However,if the native or PLL-covered DPPC/DPPG-liposomes were heated to 54 °C (lipids are transferred to the fluid state with an accompanying reduction in the vesicle size,as was previously described) a significant release is observed (profiles 6 and 7,respectively).We propose that the higher membrane permeability of the liposomes in the fluid state compared to the solid is due to the presence of defects in the fluid membrane or to a transient fragmentation that must occur during the vesicle size reduction.The observed different permeability is in agreement with studies on DPPC-based vesicle ion permeability in different phase states [39].Our
experimentsreveal that PLL-covered“fluid”vesicles release the dyefaster,indicating a direct effect of bound PLL molecules on membrane permeability (Fig.5A,profile 7).At the same time we suppose that PLL molecules do not translocate across lipid membrane because just short polypeptides are able to cross the membranes .
![英语翻译CF release from native DPPC/DPPG-liposomes and POPC/POPG](/uploads/image/z/18168822-54-2.jpg?t=%E8%8B%B1%E8%AF%AD%E7%BF%BB%E8%AF%91CF+release+from+native+DPPC%2FDPPG-liposomes+and+POPC%2FPOPG)
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搞笑吧.这么专业的文献让网友翻译.如果你是搞这一行的应该就能看懂啊.
Journal of Controlled Release
Volume 117,Issue 1,22 January 2007,Pages 111-120
Coating of negatively charged liposomes by polylysine:Drug release study
Dmitry Volodkina,,,Helmuth Mohwaldb,Jean-Claude Voegela and Vincent Balla
搞笑吧.这么专业的文献让网友翻译.如果你是搞这一行的应该就能看懂啊.
Journal of Controlled Release
Volume 117,Issue 1,22 January 2007,Pages 111-120
Coating of negatively charged liposomes by polylysine:Drug release study
Dmitry Volodkina,,,Helmuth Mohwaldb,Jean-Claude Voegela and Vincent Balla
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英语翻译要用上native